，主要包括对假阴道和输精管。用75％的酒精棉球对假阴道内外进行，待酒精挥发完毕后用。输精管时将两端分别浸入大于75％的酒精中，大约20分钟后取出，让酒精完全挥发干净后用配好的稀释液冲洗3次，放人已的大试管中待用。
Disinfection, including the vagina and vas deferens. To disinfect the vagina inside and outside with 75% alcohol cotton ball, until the alcohol evaporates after use. The vas deferens disinfection ends respectively in greater than 75% of the alcohol, about 20 minutes, let the complete evaporation of alcohol clean after diluted with a good rinse 3 times, then put in sterilized test tube.

    稀释液的配制。用10％的淡奶粉作为精液稀释液，具体配制方法是用天平(质量0．10克)称取所需的奶粉，用蒸馏水调整到所需的浓度10％，将配制好的悬浮液、纱布及若干三角瓶用蒸汽高温20分钟，然后将悬浮液用纱布过滤到三角瓶中涂室温，每毫升加入青、链霉素各1 000单位，搅匀即可。
Dilute solution. With 10% fresh milk as semen dilution, the specific preparation method is to use the balance (mass 0.10 g) were required for milk powder, distilled water is adjusted to the required concentration of 10%, the prepared suspension, gauze and a plurality of triangular bottle steam sterilized at high temperature for 20 minutes, then the gauze filter to the river the bottle is coated to room temperature by the suspension, per ml add green, streptomycin international units 1000, mix well.
    用生理盐水将结晶状的“促排3号”或“2号”溶液稀释，随用随配。奶棉的制作，用剪刀将医用脱脂棉剪成2厘米见方的小方块若干，放人大培养皿中，一起进行蒸汽高温后，倒人过滤后的淡奶粉液，凉室温备用。常用0．90％氯化钠或5％葡萄糖溶液稀释精液，稀释倍数一般为9倍。稀释液的温度应与精液等温。稀释液注入精瓶的速度不能过快，注入后再用玻璃棒轻轻搅动，使其均匀混合，稀释后应再作一次显微镜检查，发现精子活力不强或死亡精子过多则不能用。
With the physiological saline to crystalline "Pro ranked No. 3" or "2" dilute the solution, with the use with the distribution. Milk production of cotton, using scissors to the medical absorbent cotton was cut into 2 cm square. Some people, put in Petri dishes, together with high-temperature steam sterilization, into the filtered light milk liquid, cool to room temperature standby. 0.90% sodium chloride or 5% glucose solution diluted semen dilution, generally 9 times. Dilution of semen and isothermal temperature. Speed diluent injection bottles can not be too fast, then stir gently into the glass rod, so that it is evenly mixed, dilution should be make a microscopic examination, found in the sperm vitality is not strong or dead sperm too much is not used.
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